Abstract: Objective:To analyze the factors affecting hand hygiene and investigate current condition of hand hygiene of clinicians.Methods:Before physical examination or invasive operation,samples were collected from 66 clinicians,and recognition of hand hygiene was estimated through questionnaire survey of the 66 clinicians at the same time.Results:Of all the 66 samples,disqualified rate was 69.7% (46 samples) and qualified rate 30.3%;all the 66 questionnaires were collected,and it revealed that hand washing rate of aseptic operation was 27% and the one of physical examination was 11% before contacting patients;the one of no touching patients' blood or humor was 20% and the one of touching it was 100% after contacting patients.Conclusions:In order to cut off the approach associated with hand hygiene through which infections were transmitted,it's necessary to strengthen training on hand washing of clinicians,improve recognition of hand hygiene and the facilities of hand washing,and perfect surveillance as well as feedback.