Abstract: Objective: To evaluate the significance of the expressions of nuclear factor-κB(NF-κB),Cyclin D1,C-Src and EGFR in esophageal cancer.Methods: Immunohistochemistry(S-P) method was employed to determine the expressions of NF-κB,Cyclin D1,C-Src and EGFR in esophageal carcinogenesis.Results: NF-κB was not expressed in normal epithelial,but highly expressed in carcinoma tissues;and the expression level was related to lymph node metastasis(P<0.01),but not to the differentiation and depth of invasion(P>0.05);Cyclin D1 was slightly expressed in normal squamous epithelium,but highly expressed in carcinoma tissues(P<0.05);and the expression level was significantly relevant to the histology grading and metastasis of lymph nodes(P<0.01).C-Src was slightly expressed in normal squamous epithelium,but highly expressed in carcinoma tissues;and the expression level was obviously related to the differentiation,the depth of invasion and the metastasis of lymph nodes(P<0.01).EGFR protein expression in carcinoma tissues was obviously higher than that in normal squamous epithelium(P<0.01);and the expression level was significantly related to the differentiation,the depth of invasion and the metastasis of lymph nodes(P<0.05 to P<0.01).The expression of NF-κB protein was related to that of Cyclin D1,C-Src and EGFR(P<0.01) in esophageal squamous cell carcinoma.Conclusions: NF-κB may be involved in the genesis and metastasis of esophageal squamous cell carcinoma;Cyclin D1,C-Src and EGFR may participate in the genesis and development of esophageal squamous cell carcinoma;the molecular mechanism by which NF-κB facilitates the genesis and development of tumor may be related to Cyclin D1,C-Src and EGFR.