张莉, 张成书, 王君, 李旭文, 葛树星, 李光早, 林晓曦. 基因芯片技术筛选血管瘤发生相关基因[J]. 蚌埠医学院学报, 2008, 33(6): 668-671.
    引用本文: 张莉, 张成书, 王君, 李旭文, 葛树星, 李光早, 林晓曦. 基因芯片技术筛选血管瘤发生相关基因[J]. 蚌埠医学院学报, 2008, 33(6): 668-671.
    ZHANG Li, ZHANG Cheng-shu, WANG Jun, LI Xu-wen, GE Shu-xing, LI Guang-zao, LIN Xiao-xi. Identification of differentially expressed genes in proliferating hemangiomas by cDNA microarray analysis of gene expression profiles[J]. Journal of Bengbu Medical College, 2008, 33(6): 668-671.
    Citation: ZHANG Li, ZHANG Cheng-shu, WANG Jun, LI Xu-wen, GE Shu-xing, LI Guang-zao, LIN Xiao-xi. Identification of differentially expressed genes in proliferating hemangiomas by cDNA microarray analysis of gene expression profiles[J]. Journal of Bengbu Medical College, 2008, 33(6): 668-671.

    基因芯片技术筛选血管瘤发生相关基因

    Identification of differentially expressed genes in proliferating hemangiomas by cDNA microarray analysis of gene expression profiles

    • 摘要: 目的:利用基因芯片技术寻找血管瘤与正常组织之间差异表达的基因,初步探索血管瘤的发生机制。方法:将14112条cDNA用点样仪点在特制玻片上制备成表达谱芯片,将来自同一患儿的血管瘤组织和正常组织的mRNA分别逆转录为cDNA,标记Cy3和Cy5两种荧光,制备成cDNA探针,与表达谱芯片杂交,通过计算机扫描、数据处理筛选出差异表达的基因。结果:血管瘤组织与正常组织共有249个差异表达基因,其中下调基因122个,上调基因127个。上调基因主要与细胞增生、血管形成、免疫细胞的黏附、细胞周期相关蛋白等有关;下调基因主要与细胞凋亡、肿瘤抑制等有关。结论:细胞增生相关基因的上调与凋亡相关基因的下调,导致细胞增生与凋亡失调,可能是引起血管瘤发病的原因。

       

      Abstract: Objective: To investigate the differentially expressed genes of proliferating hemangiomas and normal tissue by cDNA microarray analysis of gene expression profiles and to identify the key disease related genes.Methods: Samples were processed from total RNA and purified to mRNA,which was reverse transcripted and hybridized onto Biodoor Genechip expression microarrays.Gene expression analysis were performed to determine the differences between the proliferating hemangiomas and the normal tissue.Results: In proliferating hemangioma,122 genes were up-regulated and 127 genes were down-regulated.Those over-expressed gene were mainly related to cell proliferation,angiogenesis,and immune cell adhesion,cell cycle-related protein,while the less-expressed genes were mainly related to cell apoptosis and tumor suppression.Conclusions: Over-expression of genes related to cell proliferation and less-expression of cell apoptosis related genes lead to imbalance between cell proliferation and apoptosis,which probably lead to the occurrence of hemangiomas.

       

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