游牧, 刘文, 饶圣宏, 徐振山, 宋礼华. 应用组织芯片技术检测卵巢癌组织中基质金属蛋白酶-2和p185HER-2/neu的表达及其临床意义[J]. 蚌埠医学院学报, 2011, 36(5): 448-451.
    引用本文: 游牧, 刘文, 饶圣宏, 徐振山, 宋礼华. 应用组织芯片技术检测卵巢癌组织中基质金属蛋白酶-2和p185HER-2/neu的表达及其临床意义[J]. 蚌埠医学院学报, 2011, 36(5): 448-451.
    YOU Mu, LIU Wen, RAO Sheng-hong, XU Zhen-shan, SONG Li-hua. Clinical significances of matrix metalloproteinase-2, and p185HER-2/neu expressions in ovarian cancer by tissue microarray[J]. Journal of Bengbu Medical College, 2011, 36(5): 448-451.
    Citation: YOU Mu, LIU Wen, RAO Sheng-hong, XU Zhen-shan, SONG Li-hua. Clinical significances of matrix metalloproteinase-2, and p185HER-2/neu expressions in ovarian cancer by tissue microarray[J]. Journal of Bengbu Medical College, 2011, 36(5): 448-451.

    应用组织芯片技术检测卵巢癌组织中基质金属蛋白酶-2和p185HER-2/neu的表达及其临床意义

    Clinical significances of matrix metalloproteinase-2, and p185HER-2/neu expressions in ovarian cancer by tissue microarray

    • 摘要: 目的:研究基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和p185HER-2/neu在卵巢癌组织中的表达及临床意义,探讨MMP-2和p185HER-2/neu联合和单一检测对卵巢癌诊断的价值。方法:利用组织芯片技术结合免疫组织化学SP法检测80例卵巢癌组织及30例正常卵巢组织MMP-2和p185HER-2/neu的表达。结果:MMP-2和p185HER-2/neu在卵巢癌中的阳性率均明显高于正常卵巢组织(P<0.01),MMP-2和p185HER-2/neu的阳性率与分化程度、临床分期及淋巴结转移的差异均有统计学意义(P<0.05~P<0.01),而与组织学类型无明显关系。联合MMP-2和p185HER-2/neu诊断卵巢癌的灵敏度、准确性有所上升,阴性似然比下降。结论:MMP-2和p185HER-2/neu在卵巢癌组织中的表达对卵巢癌的生长、浸润及转移起重要作用,与卵巢癌的分化、临床分期及淋巴结转移相关。

       

      Abstract: Objective: To investigate the relationship of matrix metalloproteinase-2(MMP-2) and p185HER-2/neu protein expressions in the development of ovarian cancer and to assess the value of MMP-2 and p185HER-2/neu for diagnosing and differentiating.Methods: The expressions of MMP-2 and p185HER-2/neu protein of 80 cases of ovarian cancer and 30 cases of normal tissues were examined by immunohistochemistry on tissue microarrays.Results: In ovarian cancer tissue and normal tissue,the positive expression rates of MMP-2 and p185HER-2/neu were significant differences between the two groups(P<0.01).The expressions of MMP-2 and p185HER-2/neu were closely correlated with histological grade,FIGO stage,lymph node(P<0.05-P<0.01),but no association was observed with the clinical stage.There was a significant positive correlation between the expressions of MMP-2 and p185HER-2/neu in ovarian cancer(P<0.01).Combined detection of MMP-2 and p185HER-2/neu,the sensitivity and accuracy were increased and negative likelihood ratio was decreased.Conclusions: MMP-2 and p185HER-2/neu expression might strengthen the invasion and metastasis of ovarian cancer.MMP-2 may associate with regulation of p185HER-2/neu.Joint detection of MMP-2 and p185HER-2/neu will increase the specificity of immunoassay,accuracy and expected positivity.They may be valueable indicators in detection of ovarian cancer.

       

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