水提法和微波助提法在蛹虫草多糖提取中的应用

Application of water extraction and microwave-assisted extraction method in the extraction of polysaccharides in cordyceps militaris mycelia

摘要: 目的:探讨蛹虫草菌丝体多糖提取方法的应用。方法:利用水提法和微波助提法提取蛹虫草菌丝体中多糖成分,利用苯酚-硫酸法测定其多糖的含量。结果:水提法粗多糖的提取率为22.26%,微波助提法的提取率为15.16%。结论:菌丝体粗多糖提取过程中应用传统水提法提取率高,但其工艺复杂、耗时,微波助提法工艺简便、快速。

Abstract: Objective:To investigate the method applied in the extraction of polysaccharides in Cordyceps militaris mycelia. Methods:Water-extraction method and microwave-assisted extraction method were applied in the extraction of polysaccharides. The content of polysaccharides in mycelia was determined by phenol-vitriol method. Results:The coarse polysaccharides extractive rate of waterextraction method was 22.26% in Cordyceps militaris mycelia,and the rate of water-extraction method was 15.16%. Conclusions:The extractive rate of water-extraction method is higher than the rate of microwave-assisted extraction method. But the technology of waterextraction method was complex and time consuming. The technology of microwave-assisted extraction method was simple and speedy.