JAKs家族基因在急性髓系白血病中的表达与预后的相关性分析

周黎黎; 耿英华; 朱俊锋

JAKs家族基因在急性髓系白血病中的表达与预后的相关性分析

The expression of JAKs family-gene in acute myeloid leukemia patients and its correlation with the prognosis

摘要

摘要: 目的: 分析JAKs家族基因在不同时期急性髓系白血病(AML)细胞中的表达及其与预后的相关性。分析JAKs家族基因在不同时期急性髓系白血病(AML)细胞中的表达及其与预后的相关性。方法: RT-PCR法分别检测AML初治组、复发组、完全缓解组(CR组)以及正常对照组(NC组)骨髓MNC细胞中JAKs家族基因(JAK1、JAK2、JAK3和TYK2 mRNA)的表达。同时对初治组患者随访3年, 统计3年生存率。结果: 初治组和复发组AML患者JAK3和TYK2 mRNA表达水平均高于CR组和NC组(P< 0.05~P< 0.01), 4组JAK2 mRNA表达水平差异有统计学意义(P< 0.05), 而初治组和复发组以及CR组和NC组间JAKs家族基因表达水平差异无统计学意义(P>0.05);JAK1、JAK2、JAK3和TYK2阳性组和阴性组间CR率差异无统计学意义(P>0.05)。Kaplan-Meier分析显示, JAK1、JAK2、JAK3和TYK2 mRNA阳性表达组3年生存率依次为36.75%、33.12%、22.13%和19.07%, 而阴性表达者依次为46.56%、41.25%、39.55%和37.02%。COX风险回归分析显示,JAK3和TYK2 mRNA阳性表达是影响AML患者预后的独立危险因素(P< 0.05)。结论: JAKs家族基因在AML发生、发展中发挥重要作用, 尤其是JAK3和TYK2 mRNA表达阳性与CR和总体生存率关系密切, 直接影响预后。

Abstract: Objective: To analyze the expressions of JAKs family-gene in different periods of acute myeloid leukemia(AML) cells,and its correlation with the prognosis of patients.Methods: The mRNA expressions of JAKs family-gene(including JAK1,JAK2,JAK3 and TYK2) in bone marrow MNC of the first-treated group,recurrence group and complete remission group(CR group) and normal control group(NC group) were detected using RT-PCR method.All cases were followed up for 3 years,and the 3-year survival rate was analyzed.Results: The expression levels of the JAK3 and TYK2 mRNA in the first-treated group and recurrence group were higher than those in CR group and NC group(P<0.05 to P<0.01),the differences of the expression level of the JAK2 mRNA in the 4 groups were were statisticallg significant(P<0.05),and the differences of the mRNA expression levels of JAKs family-gene between the first-treated group,recurrence group,CR group and NC group were not statistically significant(P>0.05).The differences of the CR rate in the JAK1,JAK2,JAK3 and TYK2 positive group and negative group were not statistically significant(P>0.05).Kaplan-Meier analysis showed that the 3-year survival rates in the JAK1,JAK2,JAK3 and TYK2 mRNA positive expression groups were 36.75%,33.12%,22.13% and 19.07%,respectively;the 3-year survival rates in the JAK1,JAK2,JAK3 and TYK2 mRNA negative expression groups were 46.56%,51.25%,39.55% and 37.02%,respectively.COX regression analysis showed that the positive expressions of JAK3 and TYK2 mRNA were the independent risk factors affecting the prognosis of AML patients.Conclusions: JAKs family genes play an important role in the occurrence and development of AML,especially the positive expressions of JAK3 and TYK2 mRNA are closely connected with CR and overall survival,and directly affect the prognosis.

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