FEZ1/LZTS1基因在膀胱移行细胞癌中的表达及意义

常睿; 李庆文; 关超; 周文生

FEZ1/LZTS1基因在膀胱移行细胞癌中的表达及意义

Expression and clinicopathologic significance of FEZ1/LZTS1 mRNA in bladder transitional cell carcinomas

摘要

摘要: 目的:探讨FEZ1/LZTS1基因在膀胱移行细胞癌中的表达及在肿瘤发生、发展中的作用。方法:对40例膀胱移行细胞癌和10例正常膀胱黏膜应用RT-PCR方法检测FEZ1/LZTS1基因mRNA的表达。结果:FEZ1/LZTS1基因mRNA在正常膀胱黏膜中阳性率100%,无表达缺失;而在膀胱移行细胞癌中阳性率42.50%,阴性率57.50%,两者间阴性率的差异有统计学意义(P<0.01);FEZ1/LZTS1基因mRNA在Ⅰ、Ⅱ、Ⅲ级膀胱移行细胞癌中的阴性率分别28.57%、61.54%和84.62%,三者之间阴性率差异有统计学意义(P<0.05);在表浅性和浸润性膀胱移行细胞癌中阴性率分别为28.57%和89.47%,两者之间阴性率差异亦有统计学意义(P<0.01)。结论:FEZ1/LZTS1基因mRNA在膀胱移行细胞癌中出现表达缺失,并且与肿瘤的病理分级和临床分期有关。FEZ1/LZTS1基因表达缺失可能作为评价肿瘤分化程度、估计预后的参考指标。

Abstract: Objective: To investigate the expression of FEZ1/LZTS1 mRNA and evaluate its effects on the tumorigenesis and progression in bladder transitional cell carcinomas (BTCCs).Methods: The expression of FEZ1/LZTS1 mRNA in 40 BTCCs and 10 normal bladder mucosas was detected by RT-PCR.Results: The negative expression of FEZ1/LZTS1 mRNA in BTCC (57.50%) was significantly higher than that in normal bladder mucosa (P<0.01).Statistical analysis showed a significant association between the Absence of FEZ1/LZTS1 mRNA and the histological grade of BTCC.The negative expression of FEZ1/LZTS1 mRNA was 28.57%,61.54% and 84.62% in gradeⅠ,gradeⅡ and grade Ⅲ BTCC,respectively (P<0.05).The negative expression was 28.57% and 89.47% in Ta-T1 BTCC and T2-T4 BTCC,respectively (P<0.01).Conclusions: The Absence of FEZ1/LZTS1 mRNA expression in BTCC is up-regulated and associated with the histological grade and the clinical stages of BTCC.The Absence of FEZ1/LZTS1 expression might act as a reference indicator in evaluating the differentiation of the tumor and predicting the prognosis.

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