Abstract: Objective: To explore the change rule of bone gla-protein(BGP),calcitionin(CT),parathormone(PTH) and β₂-microglobulin(β₂-BG) in serum of 30 patients with chronic renal disease(CRF) by different hemodialysis methods,and compare the therapy effect between low-flux hemodialysis(LFHD) and high-flux hemodialysis(HFHD).Methods: Thirty cases of chronic hemodialysis patients were divided into HFHD group(12 cases) and LFHD group(18 cases),they were followed up for 4 weeks.The variation of serum levels of BGP,CT,PTH,β₂-MG in CRF patients were analyzed by radioimmunoassay and chemiluminescent detection methods before and 4 week after hemodialysis.Results: The levels of BGP,PTH,β₂-MG and P^3- in serum of patients with CRF were higher than those of the controls(P<0.01),but the levels of CT and Ca²⁺ were lower than that of the controls(P<0.01 and P<0.05) significantly;the level of β₂-MG was increased in LFHD group while decrease in HFHD group,and the levels of BGP and PTH were decreased significantly in HFHD group(P<0.01).CT level was increased in LFHD group while decreased in HFHD group(P<0.01).The levels of PTH and β₂-MG were markedly reduced when post-dialysis and 4 weeks after dialysis comparing to those of pre-dialysis(P<0.01).The symptoms such as shin pitch,arthralgia,muscle spasm and so on in HFHD group were significantly ameliorated.Conclusions: There was a significant clinical value in testing serum BGP,CT,PTH and β₂-MG concentrations for analyzing patients condition,assessing the treatment effect and estimating prognosis of patients with CRF.The efficacy of HFHD is better than that of LFHD.