老年急性心肌梗死病人外周血单核细胞中Tim-3、miR-326表达及与预后的关系

王素荣; 刘美华; 李建霞

老年急性心肌梗死病人外周血单核细胞中Tim-3、miR-326表达及与预后的关系

Study on the relationship between the expression levels of Tim-3 and miR-326 in peripheral blood mononuclear cells of elderly patients with acute myocardial infarction and with prognosis

摘要

摘要:
目的： 分析老年急性心肌梗死（AMI）病人外周血单核细胞（PBMC）中T淋巴细胞免疫球蛋白黏蛋白分子3（Tim-3）、微小RNA-326（miR-326）表达水平及二者与病人预后的关系。
方法： 选取发病12 h内、且急诊行PCI治疗的AMI病人116例为AMI组，另选择同期体检健康者110例为对照组。采用荧光定量PCR法分析PBMC中Tim-3、miR-326的水平；Pearson法分析AMI病人PBMC中Tim-3、miR-326水平的相关性；多因素logistic回归分析AMI病人主要不良心血管事件（MACEs）发生的影响因素。
结果： AMI组治疗前PBMC中Tim-3水平高于对照组及治疗后，miR-326水平低于对照组及治疗后（P ＜ 0.05）。AMI病人PBMC中Tim-3与miR-326呈负相关关系（P ＜ 0.05）。Tim-3高表达组MACEs总发生率高于Tim-3低表达组，miR-326高表达组MACEs总发生率低于miR-326低表达组（P ＜ 0.05）。发生MACEs病人PBMC中Tim-3水平及Gensini积分、心肌肌钙蛋白水平、心肌梗死面积均明显高于未发生MACEs病人，PBMC中miR-326水平明显低于未发生MACEs病人（P ＜ 0.05）。Gensini积分、心肌肌钙蛋白、Tim-3是AMI病人MACEs发生的独立危险因素，miR-326是AMI病人MACEs发生的保护因素（P ＜ 0.05）。
结论： PBMC中Tim-3、miR-326水平对评估AMI病人PCI术后MACEs发生率有一定价值。

Abstract:
Objective To investigate the expression levels of T lymphocyte immunoglobulin mucin molecule 3(Tim-3) and microRNA326 (miR-326) in peripheral blood mononuclear cells (PBMCs) of elderly patients with acute myocardial infarction (AMI), and their relationship with the prognosis of patients.
Methods A total of 116 AMI patients treated with PCI within 12 hours of onset were selected as the AMI group, and 110 healthy subjects were selected as control group at the same time. The levels of Tim-3 and miR-326 in PBMC were analyzed by fluorescence quantitative PCR, the Pearson method was used to analyze the correlation between the levels of Tim-3 and miR-326 in PBMCs of patients with AMI, and the multivariate logistic regression was used to analyze the influencing factors of major adverse cardiovascular events (MACEs) in patients with AMI.
Results The level of Tim-3 in PBMC of AMI group before treatment was higher than that of control group and after treatment, and the level of miR-326 was lower than that of control group and after treatment (P ＜ 0.05). There was a negative correlation between Tim-3 and miR-326 in PBMC of AMI patients (P ＜ 0.05). The total incidence rate of MACEs in Tim-3 high expression group was higher than that in Tim-3 low expression group, and the total incidence rate of MACEs in miR-326 high expression group was lower than that in mir-326 low expression group (P ＜ 0.05). The levels of Tim-3, Gensini score, cardiac troponin and myocardial infarction area in PBMC of patients with MACEs were significantly higher than those of patients without MACEs, and the levels of miR-326 in PBMC of patients with MACEs were significantly lower than those of patients without MACEs (P ＜ 0.05). The Gensini score, cardiac troponin and Tim-3 were the independent risk factors of MACEs in patients with AMI, while miR-326 was a protective factor of MACEs in patients with AMI (P ＜ 0.05).
Conclusions The levels of Tim-3 and mir-326 in PBMC have certain value in evaluating the incidence of MACEs in patients with AMI after PCI.

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