王慧, 吴俊英. 靶向4-1BBL基因siRNA抑制HL-60B细胞生长的体外研究[J]. 蚌埠医学院学报, 2010, 35(8): 757-761.
    引用本文: 王慧, 吴俊英. 靶向4-1BBL基因siRNA抑制HL-60B细胞生长的体外研究[J]. 蚌埠医学院学报, 2010, 35(8): 757-761.
    WANG Hui, WU Jun-ying. 4-1BBL small interfering RNA inhibits the proliferation of HL-60B cell in vitro[J]. Journal of Bengbu Medical College, 2010, 35(8): 757-761.
    Citation: WANG Hui, WU Jun-ying. 4-1BBL small interfering RNA inhibits the proliferation of HL-60B cell in vitro[J]. Journal of Bengbu Medical College, 2010, 35(8): 757-761.

    靶向4-1BBL基因siRNA抑制HL-60B细胞生长的体外研究

    4-1BBL small interfering RNA inhibits the proliferation of HL-60B cell in vitro

    • 摘要: 目的:观察4-1BBL基因小分子干扰RNA(siRNA)对HL-60细胞生长及诱导其凋亡作用的影响。方法:化学合成法合成靶向人4-1BBL基因siRNA,脂质体转染法转染HL-60B细胞;半定量PCR和流式细胞术检测转染前后HL-60B细胞4-1BBL的表达水平,MTT法检测HL-60B细胞增殖,流式细胞术检测细胞凋亡。结果:靶向4-1BBL基因siRNA转染HL-60B细胞后,4-1BBLmRNA和蛋白的表达明显降低;细胞增殖被抑制,其抑制作用在转染后48h最明显,增生抑制率达到(22.1±2.3)%;细胞凋亡率增加,由(9.8±2.5)%上升到(32.5±5.1)%。结论:化学合成的siRNA在体外能成功抑制靶基因4-1BBL的表达和HL-60B细胞的增殖,并有助于细胞趋向凋亡。

       

      Abstract: Objective: To observe the influence of 4-1BBL small interfering RNA (siRNA) on the growth and apoptosis of HL-60B cells.Methods: The siRNA for 4-1BBL gene was designed and synthesized in vitro,and then transfected into HL-60B cells by Lipofectamine;the expression of 4-1BBL mRNA and protein was detected by RT-PCR and flow cytometry,respectively;the proliferative rate of HL-60B cells was determined by methyl thiazoy tetralium (MTT),and the cell apoptosis was analyzed by flow cytometry.Results: After the siRNA for 4-1BBL gene was transfected into HL-60B cells,the expression of 4-1BBL gene and protein decreased,the growth of cells was repressed and the proliferation rate of HL-60B cells decreased.The inhibition rate was (22.1±2.3)% 48 h after transfection;the apoptosis rate increased from (9.8±2.5)% to (32.5±5.1)%.Conclusions: Chemically synthetic siRNA can decrease 4-1BBL gene expression,inhibit cellular proliferation and promote apoptosis in vitro.

       

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