Abstract: Objective: To investigate the effects of transcription factors ETS-1 on the proliferation of the breast cancer cells by regulating miR-96.Methods: The expression of miR-96 in breast cancer cell MCF-7 with silencing the ETS-1 expression induced by the RNA interference technique was detected.The binding site of transcription factor ETS-1 was predicted using bioinformatics method,and validated with chromatin immune precipitation reaction.The miR-96 was transfected into breast cancer cell MCF-7,and the effect of which on the proliferation and clone information of breast cancer cells was observed.Results: The expression of miR-96 in breast cancer cells was significantly increased after inhibiting the expression of ETS-1(P<0.01).The expression of ETS-1 was regulated by the ETS-1 binding to the promoter region of miR-96.Compared with the control group,the abilities of proliferation and clone formation of MCF-7 were significantly increased after the overexpression of miR-96(P<0.01).Conclusions: ETS-1 can affect the proliferation and clone formation ability of breast cancer cells by regulating the expression of miR-96,which is expected to become a new target in the treatment of breast cancer.