Abstract: Objective: To investigate the association of subjective well-being with self-concordance and personality of the medical graduates. Methods: A total of 332 medical graduates were surveyed by General Well-Being Schedule,Self Consistency and Congruence Scale,and Eysenck Personality Questionnaire. Results: The scores of the average general well-being turned out to be 79.76 ±12.76 and self-consistency 89.56 ±13.41; differences existed in students of different genders and different grades (P < 0.05 to P < 0.01); no distinct differences were observed in personality types (P > 0.05). Self-stereotypy,lack of self-experiences,psychoticism and nervosity were negatively related to the general well-being (P < 0.01),while self-flexibility and extroverted personality were positively related to the general well-being (P < 0.01). Conclusions: Self-flexibility and stable personality trait are important factors affecting the general well-being of the medical graduates.