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    血管紧张素Ⅱ对肾小管上皮细胞分泌纤连蛋白的影响

    Effect of angiotensin Ⅱ on fibronectin biosynthesis in human renal tubular epithelial cell

      摘要
    • 摘要: 目的:观察血管紧张素Ⅱ(AngⅡ)对体外培养的肾小管上皮细胞分泌细胞外基质成分纤连蛋白的影响。方法:采用免疫印迹法(Westernblot)及细胞免疫荧光法检测10-6mol/LAngⅡ分别刺激肾小管上皮细胞6、12、24、48、72、96h,以及不同浓度(10-6、10-7、10-8mol/L) AngⅡ刺激96h时细胞纤连蛋白的表达。结果:10-6mol/LAngⅡ刺激体外培养的肾小管上皮细胞12h后纤连蛋白表达开始增加,72h达高峰。不同浓度的AngⅡ刺激96h后,纤连蛋白的表达均有增加,10-6mol/L增加最明显,10-7、10-8mol/L次之。结论:AngⅡ能使体外培养的肾小管上皮细胞分泌纤连蛋白增加,并具有时间和浓度依赖性。

       

      Abstract: Objective:To investigate the effect of angiotensin Ⅱ(AngⅡ) on fibronectin(FN) biosynthesis on human renal tubular epithelial cells.Methods:Cultured human renal tubular epithelial cells were treated with AngⅡ at the concentrations of 10-6 mol/L for 6,12,24,48,72 and 96 hours;and the cells were treated with AngⅡ at the concentrations of 10-8,10-7 and 10-6 mol/L for 96 hours.The FN expression in renal tubular cells was determined by both Western blot and immunofluorescence.Results:Fibronectin synthesis was increased by AngⅡ at a concentration of 10-6 mol/L in the renal tubular epithelial cells.The expression of FN started to increase after 12 hours,and reached the peak after 72 hours.The synthesis of FN was the most obvious at a concentration of 10-6 mol/L,and the following was at 10-7 and 10-8 mol/L.Conclusions:It suggests that AngⅡ stimulates FN secretion of human renal tubular epithelial cells in a dose and time-dependent manner.

       

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