血清FOXP3、PTX3在自身免疫性卵巢早衰病人表达及其与性激素水平的关系

郝冬梅; 于月新; 赵莹莹

血清FOXP3、PTX3在自身免疫性卵巢早衰病人表达及其与性激素水平的关系

The expression of serum FOXP3 and PTX3 in patients with autoimmune premature ovarian failure and their relationship with sex hormone levels

摘要

摘要:
目的： 探讨自身免疫性卵巢早衰（APOF）病人血清中叉头状转录因子P3（FOXP3）、正五聚蛋白（PTX3）的表达水平以及二者与性激素水平之间的关系。
方法： 选取确诊为卵巢早衰的126例病人为研究对象，将其中60例APOF病人设置为观察组，另66例非APOF病人为对照组，同时将体检的66例无卵巢早衰的健康女性设置为健康组。三组血清中FOXP3 mRNA的相对表达水平采用实时荧光定量PCR（qRT-PCR）法检测，PTX3的表达水平以及卵泡刺激素（FSH）、雌二醇（E2）、黄体生成素（LH）等性激素水平采用酶联免疫吸附法测定。APOF病人血清中FOXP3 mRNA、PTX3的表达与雌激素水平的相关性采用Pearson法进行分析；采用logistic回归分析病人发生APOF的影响因素；ROC曲线分析血清FOXP3、PTX3对APOF的诊断价值。
结果： 观察组血清中FOXP3 mRNA表达量显著低于对照组和健康组（P ＜ 0.05），PTX3表达水平显著高于对照组和健康组（P ＜ 0.05）。观察组血清FSH水平显著高于对照组和健康组（P ＜ 0.05），血清E2、LH水平显著低于对照组和健康组（P ＜ 0.05）。APOF病人血清FOXP3 mRNA与性激素FSH呈负相关关系（P ＜ 0.05），与性激素E2、LH呈正相关关系（P ＜ 0.05）；PTX3表达与FSH呈正相关关系（P ＜ 0.05），与E2、LH呈负相关关系（P ＜ 0.05）。FOXP3、E2水平是影响APOF发生的保护因素（P ＜ 0.05），而PTX3、FSH水平是影响APOF发生的危险因素（P ＜ 0.05）。血清FOXP3、PTX3两者联合预测APOF效果更好。
结论： FOXP3 mRNA在APOF病人血清中低表达，PTX3在APOF病人血清中高表达，二者表达水平均与病人性激素水平具有相关性，且与病人发生APOF密切相关。

Abstract:
Objective To investigate the expression levels of forkhead box protein 3 (FOXP3) and pentaxin-3 (PTX3) in the serum of patients with autoimmune premature ovarian failure (APOF) and their relationship with sex hormone levels.
Methods A total of 126 patients diagnosed with premature ovarian failure were selected as the study subjects. Among them, 60 APOF patients were assigned to the observation group, 66 non-APOF patients served as the control group, and 66 healthy women without premature ovarian failure were included as the healthy group. The relative expression levels of FOXP3 mRNA in serum were detected using real-time quantitative PCR (qRT-PCR), while PTX3 expression levels and sex hormone levels, including follicle-stimulating hormone (FSH), estradiol (E2), and luteinizing hormone (LH), were measured by enzyme-linked immunosorbent assay. The correlation between FOXP3 mRNA and PTX3 expression in APOF patients' serum and estrogen levels was analyzed using Pearson's method. Logistic regression was employed to assess the influencing factors of APOF occurrence, and ROC curve analysis was conducted to evaluate the diagnostic value of serum FOXP3 and PTX3 for APOF.
Results The serum FOXP3 mRNA expression level in the observation group was significantly lower than that in the control group and healthy group (P ＜ 0.05), while the PTX3 expression level was significantly higher than that in the control group and healthy group (P ＜ 0.05). The serum FSH level in the observation group was significantly higher than that in the control group and healthy group (P ＜ 0.05), whereas the serum E2 and LH levels were significantly lower (P ＜ 0.05). FOXP3 mRNA in APOF patients' serum showed a negative correlation with FSH (P ＜ 0.05) but a positive correlation with E2 and LH (P ＜ 0.05). PTX3 expression was positively correlated with FSH (P ＜ 0.05) and negatively correlated with E2 and LH (P ＜ 0.05). FOXP3 and E2 levels were protective factors influencing APOF occurrence (P ＜ 0.05), while PTX3 and FSH levels were risk factors (P ＜ 0.05). Combined prediction of FOXP3 and PTX3 in serum demonstrated better efficacy for APOF diagnosis.
Conclusions FOXP3 mRNA is lowly expressed in the serum of APOF patients, while PTX3 is highly expressed. Both expression levels are correlated with sex hormone levels and closely associated with the occurrence of APOF.

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