Abstract: Objective: To study the value of 12 tumor markers in protein chip system for multi-tumor detection(C12) in the diagnosis of the primary liver cancer.Methods: The system of C12 was used to quantitatively detect the 12 tumor markers(CA125,Ferritin,CEA,AFP,CA19-9,CA15-3,NSE,CA242,PSA,Free PSA,HGH,β-hCG) in the sera of 128 patients with primary liver cancer,23 patients with hepatocirrhosis,and 98 healthy controls.Results: The quantitative detection proved positive in 112 of the 128 patients with primary liver cancer;the sensitivity was raised from 58.6% by AFP single index detection to 87.5% and the specificity reached 83.5%.In addition,Ferritin positive rate achieved 41.4%,and in the sera of some liver cancer patients,the difference of β-hCG,NSE,PSA,HGH,CA19-9,CA125,CEA,AFP,CA15-3 and CA242 items was statistically significant,compared with the contrast group and hepatocirrhosis group(P<0.01).Conclusions: The allied detection is more effective than the traditional single index detection.