Expressions of MMP-9 and TIMP-2 in ESCC and their association with the recurrence,metastasis and prognosis of the tumor
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摘要: 目的：探讨基质金属蛋白酶-9（MMP-9）和基质金属蛋白酶抑制剂-2（TIMP-2）在食管癌中的表达及与食管癌术后复发或转移和预后之间的关系。方法：采用组织芯片技术，用免疫组织化学方法检测食管癌术后存活5年以上而无复发转移组（A组）和术后1年内发生复发转移而病死组（B组）食管鳞状细胞癌（ESCC）病例中的MMP-9、TIMP-2表达情况。结果：MMP-9、TIMP-2在正常食管鳞状上皮中不表达或少量表达，在食管癌组织中有不同程度的表达。有淋巴结转移者MMP-9阳性表达明显高于无淋巴结转移者（P<0.01）；TIMP-2表达在分化程度和有无淋巴结转移间差异均有统计学意义（P<0.05）。TIMP-2表达与MMP-9表达无相关关系（P>0.05）。结论：MMP-9、TIMP-2在食管癌组织中高表达，其表达情况与食管癌术后发生复发转移有明显的相关性，联合检测MMP-9、TIMP-2 表达对预测ESCC术后发生复发转移和预后有重要价值，可列为手术切除标本的常规检查项目，以指导术后治疗。
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关键词:
- 食管肿瘤 /
- 复发转移 /
- 基质金属蛋白酶-9 /
- 基质金属蛋白酶抑制剂-2
Abstract: Objective: To investigate the expressions of matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-2(TIMP-2) in esophageal carcinoma and their relation with the recurrence or metastasis after esophagectomy.Methods: The expressions of MMP-9 and TIMP-2 in patients with ESCC who survived over 5 years without recurrence or metastasis(group A) and those who died of recurrence or metastasis within 1 year(group B) were detected by immunohistochemistry with Tissue Chip technique.Results: There was no expression or little expression of MMP-9 and TIMP-2 in normal esophageal mucosa,but different degrees of expression in esophageal carcinoma.The expression of MMP-9 was significantly higher in patients with lymph node metastasis than in those without(P<0.01);the expression of TIMP-2 was positively correlated with the tumor differentiation and lymph node metastasis(P<0.05);the expression of MMP-9 was not correlated with the expression of TIMP-2(P>0.05).Conclusions: The MMP-9 and TIMP-2 express high in the esophageal carcinoma tissue,and the expression level is significantly correlated with the recurrence or metastasis of the tumor after esophagectomy.Determination of the expression of MMP-9 and TIMP-2 may be of prognostic value in predicting the recurrence or metastasis of the tumor.It is suggested that this detection be included in the routine examination item for the resected specimen to direct the postoperative treatment. -
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