Abstract: Objective: To summarize the experience in prevention and treatment of bile duct injury during laparoscopic cholecysteclomy(LC).Methods: Retrospective analysis on the clinical data of 30 cases of LC was carried out.The different operation modes were selected according to the injury area and degree of bile duct and the discovered time.Results: Nine cases who treated urgently were recovered well during the operation period.In the 21 cases who were found with bile duct injury after the operation,two cases were died,and in which one was died with leakage of bile and celiac infection,and another was died with reiterative cholangitis and hepatic insufficiency.Other cases were recovered well.Conclusions: During LC,the operative rule should always be strictly complied and the key to preventing bile duct injury is to avoid clipping,cutting and coagulationblindly blindly.