Objective To investigate the clinical value of serum levels of forkhead box protein O1 (FOXO1) and peroxisome proliferator-activated receptor-γ (PPAR-γ) in the diagnosis of deep venous thrombosis (DVT) of lower limbs.

Methods A total of 132 patients with lower limb disorders were selected. According to the diagnosis results of digital subtraction angiography (DSA), the patients were divided into the DVT group (87 cases) and non DVT group (45 cases). Additionally, 132 healthy physical examiners during the same period were selected as the control group. The influencing factors of DVT and correlation between serum FOXO1 and PPAR-γ expression levels in DVT patients were analyzed, and the ROC curve was used to analyze the diagnostic value of serum FOXO1 and PPAR-γ levels for DVT.

Results There were statistically significant in the PT, APTT, platelet counts and levels of FOXO1 and PPAR-γ among three groups (P ＜ 0.05). There was a negative correlation between serum FOXO1 and PPAR-γ expression levels in DVT patients (r = –0.750, P ＜ 0.05). The history of hypertension, prothrombin time (PT) ＜ 8 s, activated partial prothrombin time (APTT) ＜ 27s, platelet count ≥ 300 × 10⁹/L and high FOXO1 were the independent risk factors of DVT, while the PPAR-γ was a protective factor of DVT (P ＜ 0.05 ~ P ＜ 0.01). The area under curve (AUC) of serum FOXO1, PPAR-γ and their combined detection in the diagnosis of DVT were 0.745, 0.657 and 0.831, respectively, the combined detection of both was superior to the individual detection of serum FOXO1 and PPAR-γ (P ＜ 0.01).

Conclusions The expression levels of serum FOXO1 and PPAR-γ are closely related to the occurrence of DVT. The combined detection of FOXO1 and PPAR-γ has good diagnostic value for DVT, and can be used to assist in the diagnosis of DVT.