限制性体位对大鼠膈肌功能改变的影响

    Restrictive position affects the diaphragm function of rats

    • 摘要: 目的:研究限制性体位大鼠膈肌功能的变化。方法:SD大鼠20只随机分成对照组和限制性体位组,每组10只。限制性体位组大鼠双前肢固定悬挂12h后,应用体外灌流大鼠膈肌条的方法,分别测量其单收缩力(Pt)、最大强直张力(Po)、峰值收缩时间(CT)、半舒张时间(1/2RT),并测量刺激频率在10、20、40、60、100 Hz的张力,绘制张力-频率曲线。同时测定膈肌组织中超氧化物歧化酶(SOD)、琥珀酸脱氢酶(SDH)活性及丙二醛(MDA)含量,电镜观察膈肌组织超微结构的变化。结果:限制性体位组大鼠Pt、Po均低于对照组(P<0.01);CT、1/2RT均高于对照组(P<0.01)。给予10、20、40、60、100Hz的电压刺激膈肌时,限制性体位组大鼠膈肌张力均低于对照组(P<0.05~P<0.01)。其组织SOD、SDH活性均明显低于对照组(P<0.01),而MDA含量显著高于对照组(P<0.01)。限制性体位大鼠膈肌肌丝紊乱、断裂,线粒体空泡化或囊泡化,有髓板样物形成;肌浆网明显扩张。结论:限制性体位大鼠膈肌肌纤维结构受到破坏,收缩功能下降,可能与SOD、SDH活性降低,MDA含量增高有关。

       

      Abstract: Objective: To study the changes of diaphragm function of rats when in restrictive position.Methods: Twenty SD rats were randomly divided into two groups:control group and restrictive position group.The rats in restrictive position group were hung with their upper limbs bound for 12 hours.The maximal twitch torce(Pt),maximal tetanic torce(Po),contraction time(CT),half-relaxtion time(1/2 RT),and the force frequency curve at 10,20,40,60 and 100Hz were recorded.The superoxide dismulase(SOD),malondialdehyde(MDA),succinic dehydrogenase(SDH) in the diaphragm were detected.The ultrastructures of the diaphragm were examined by electron microscope.Results: The Pt and Po in the restrictive position group were lower than those in the control group(P<0.01),but the CT and 1/2RT were longer(P<0.01);When 10,20,40,60 and 100 Hz square-wave voltage was given to stimulate the diaphragm,the tension of the diaphragm in the restrictive position group was significantly lower than that of the control group(P<0.05 to P<0.01).The SOD and SDH in the restrictive position group were significantly lower than those of control group(P<0.01),and the MDA were higher(P<0.01).The electron microscopic morphometry of the diaphragm in the restrictive position group revealed that the diaphragm myoneme was confused and broken;the mitochondria was in a state of vacuolization and expanded;Myelinfigures could be seen,and the sarcoplasmic reticulum was distended.Conclusions: The diaphragm systolic function of the rats in the restrictive position group is damaged,which may be related to the decreased activity of SOD and SDH,as well as the increase of MDA content.

       

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