Objective To explore the construction of organoid models derived from gastric cancer patients, and conduct a preliminary assessment of the established organoid models.

Methods The fresh tissues from patients with gastric cancer during the operation were collected. The gastric cancer tissues were dissociated into single-cell suspensions by enzymatic hydrolysis, and cultured in Matrigel for 7 days. The morphological characteristics of the primary tissues of patients and corresponding PDO were compared by hematoxylin-eosin staining technique, and the expression levels of carcinoembryonic antigen (CEA) and proliferating cell nuclear antigen (Ki-67) in PDO were detected by immunohistochemical staining method.

Results A total of 4 surgical samples from gastric cancer patients were included. Three gastric cancer PDO models that could be stably passaged and cryopreserved were constructed, with a survival rate of 75%. The results of H&E staining showed that the structure of PDO was highly similar to that of the primary gastric cancer tissue. The results of immunohistochemical staining further confirmed that the expression patterns of Ki-67 and CEA in PDO were basically consistent with those in the primary gastric cancer tissue.

Conclusions A PDO model of gastric cancer that is highly consistent with the primary gastric cancer tissue at the tissue and cellular levels has been established. This model has the ability of stable passage and long-term cryopreservation, and is expected to provide a powerful experimental platform for drug screening and basic research of gastric cancer.