Objective To analyze the application value of immunoturbidimetry method for detecting CK-MB mass (CK-MBmass) in distinguishing falsely elevated CK-MB activity and in the auxiliary diagnosis of acute myocardial infarction (AMI).

Methods A total of 80 cases with CK-MB activity greater than CK total activity, 80 cases of AMI patients, and 30 cases of non-AMI patients (control group) were collected. CK-MBmass was determined by immunoturbidimetry method and chemiluminescence method, CK-MBmass by immunoturbidimetry method and enzyme activity method, and cTnI was detected by chemiluminescence immunoassay. the consistency of the above detection results was compared.

Results The immunoturbidimetry method and chemiluminescence method showed high consistency in detecting positive samples of CK-MBmass (P ＞ 0.05), while significant differences showed between the enzyme activity method and chemiluminescence method and immunoturbidimetry method (P ＜ 0.05). The positive agreement rate of CK-MBmass detection results by immunoturbidimetry method and chemiluminescence method in the AMI group was close to that of cTnI positivity. In the 80 cases of CK-MB activity and CK total activity reversed samples, the consistency of CK-MBmass detection results by immunoturbidimetry method and chemiluminescence method was also consistent.

Conclusions The detection results of CK-MB mass using immunoturbidimetric assay are generally consistent with those obtained via chemiluminescence immunoassay, demonstrating good agreement and meeting the requirements of routine clinical diagnostics. At the same time, it provides a feasible diagnostic approach for primary healthcare institutions lacking chemiluminescence analyzers in the diagnosis of acute myocardial infarction (AMI).