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    白细胞增高对血红蛋白测定结果影响的探讨

    The effect of the increasing white blood cells on the hemoglobin level

      摘要
    • 摘要: 目的: 探讨白细胞 (WBC) 增高对血红蛋白(Hb)测定结果的影响。方法: 收集住院患者血液标本90份, 其中WBC值在(10～<20)×109/L、(20～<40)×109/L、≥40×109/L的标本各30份, 分别通过XE-2100型血液分析仪和氰化高铁血红蛋白(HiCN)比色法检测各标本的Hb值, 其中HiCN比色法首先在660 nm处用分光光度计测其浊度, 然后高速离心去除WBC, 用上清液进行比色。观察2次测定Hb值的变化。结果: WBC值≥40×109/L, 会对Hb结果产生影响, 且WBC值越高, 产生的浊度越大, 对Hb的影响越明显(P<0.01)。结论: 高WBC的存在将使Hb测定值假性增高, 使用HiCN稀释血液, 高速离心去除WBC后再比色测定可有效消除WBC异常增高对Hb的影响。

       

      Abstract: Objective: To investigate the effects of the increasing of white blood cells(WBC) on the hemoglobin(Hb) level.Methods: The Hb values of 90 blood specimensWBC value for (10 to <20)×109/L in 30 cases,(20 to <40)×109/L in 30 cases and ≥40×109/L in 30 cases were determined by XE-2100 blood analyzer and the reference method of hemoglobinometry(HiCN colorimetric method).The turbidity of specimen was measured at the 660 nm wave length of spectrophotometer by HiCN colorimetric method,the WBC in specimens were removed by high-speed centrifuge,and then the Hb values in supernatant were determined by the colorimetric method.The Hb values between before and after centrifuge were compared.Results: The effects of the WBC value≥40×109/L on Hb was great.With the increase of the WBC value,the turbidity was more higher,and the effect of Hb was the more obvious(P<0.05).Conclusions: The high WBC can increase the false Hb value.The effects of the abnormal increasing of WBC on Hb value can be eliminated by the HiCN diluting blood,high-speed centrifuge to removing WBC and colorimetric assay.

       

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