Abstract: Objective: To investigate the bacterial contamination of air and articles in ambulance, and observe the effect of disinfection. Methods: The bacterial contamination of the air and articles in one ambulance were detected before and after disinfection in early,middle and end per month from May. 2009 to Oct. 2009. Results: The pollution situation of air and articles in ambulance was serious before disinfection. The bacterial contamination was decreased significantly after disinfection. And the difference was statistically significant(P < 0.01). Conclusions: The pollution situation of air and articles in ambulance is serious. It is necessary to establish a strict disinfection management system.