Abstract: Objective: To explore the clinical features of infants infected with cytomegalovirus(CMV) and investigate the detection methods and treatment.Methods: The clinical data of 25 infants with CMV infection confirmed by detection of serum CMV-IgM using enzyme immunodot technic were analyzed.Results: Of the 25 patients,bronchopneumonia accounted for 11 cases(44%),central nervous system damage 11 cases(44%)and hyperbilirubinemia 8 cases(32%).All the patients were treated with ganciclovir.Bronchopneumonia was cured in 8 cases and improved in 3 cases.Central nervous system damage was cured in 1 case,improved in (9 cases) and uncured in 1 case.Hyperbilirubinemia was cured in 2 cases and improved in 6 cases.Conclusions: The most common manifestations of CMV infection in infants include bronchopneumonia,central nerves system damage and hyperbilirubinemia.The presence of serum CMV-IgM antibody is a laboratory diagnostic evidence.Ganciclovir is the choice of treatment for CMV infection.