The effect of reduced glutathione on glomerular endothelial function in diabetic rats
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摘要: 目的:观察早期糖尿病肾病(diabetic nephropathy,DN)大鼠肾小球内皮功能的特点,探讨还原型谷胱甘肽对早期DN大鼠内皮功能的影响。方法:将大鼠分为对照组、模型组和治疗组,应用链脲佐菌素腹腔注射建立DN模型,治疗组每天腹腔注射还原型谷胱甘肽。应用相关试剂盒检测尿白蛋白(UA)、肾小球组织一氧化氮(NO)、内皮型一氧化氮合酶(eNOS)活性、血管性假性血友病因子(vWF)和超氧化物歧化酶、丙二醛(MDA)、谷胱甘肽过氧化物酶。结果:与对照组比较,模型组早期DN大鼠肾小球NO含量、eNOS活性均显著下降,vWF水平明显升高,UA显著增加(P<0.01),且UA增加的程度与vWF升高的水平呈正相关关系(P<0.05);肾小球组织中超氧化物歧化酶、谷胱甘肽过氧化物酶均明显下降,MDA明显升高(P<0.01)。与模型组比较,治疗组大鼠肾小球NO含量、eNOS活性均明显上升,vWF水平显著降低,UA明显减少,肾小球组织中MDA显著下降(P<0.01)。结论:早期DN大鼠肾小球内皮功能紊乱,氧化应激水平明显升高,还原型谷胱甘肽可能通过降低早期DN大鼠肾小球内氧化应激水平从而改善肾小球内皮功能。Abstract: Objective: To observe the feature of glomerular endothelial function in rats with early diabetic nephropathy(DN),and evaluate the effect of reduced glutathione on glomerular endothelial function in diabetic rats.Methods: Streptozotocin(60mg/ kg) was injected into peritoneal cavity in the rats to establish animal DN model.The diabetic rats were randomly divided into DN model group and reduced glutathione treated group.Coherent kits were used to detect glomerular nitrogen monoxide(NO),the activity of glomerular endothelial nitric oxide synthase(eNOS),von Willebrand factor(vWF),urinary albumin(UA),superoxide dismutase(SOD),malondialdehyde(MDA) and glutathione peroxidase.Results: Compared with normal control group,glomerular NO,activities of glomerular eNOS were significantly decreased in early DN rats,while the level of vWF and UA significantly increased(P<0.01),and the level of UA was correlated with the level of glomerular vWF(P<0.05).Glomerular SOD,glutathione peroxidase significantly decreased in early DN rats,while MDA was significantly increased(P<0.01).Compared with DN model group,glomerular NO,activities of glomerular eNOS were significantly increased in early DN rats treated with reduced glutathione,while the level of vWF and UA were significantly decreased,and glomerular MDA was significantly increased in early DN rats treated with reduced glutathione(P<0.01).Conclusions: Glomerular endothelial function is indiscriminate in early DN rats,while the level of oxidative stress significantly increases.Reduced glutathione improves glomerular endothelial dysfunction in early DN rats,maybe through reducing oxidative stress.
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