聚乙二醇预处理脂血标本前后FT<sub>3</sub>和FT<sub>4</sub>的变化

聚乙二醇预处理脂血标本前后FT₃和FT₄的变化

摘要: 目的:探讨用聚乙二醇(polyethylene glycol,PEG)预处理脂血标本前后游离三碘甲状腺原氨酸(FT₃)和游离甲状腺素(FT₄)的变化。方法:将28名正常人组和20例脂血组的血清分两份,一份直接测定,另一份用95g/LPEG液处理标本后,再以放射免疫法测定FT₃和FT₄。结果:正常人组PEG处理前后,FT₃和FT₄检测结果差异均无统计学意义(P>0.05);而脂血标本组用PEG处理前FT₃和FT₄检测结果显著高于PEG处理后(P<0.001)。结论:正常情况下,PEG处理标本不影响FT₃和FT₄检测。标本经PEG处理后,去除脂质、甲状腺激素自身抗体的干扰,提高结果的准确性。

Abstract: Objective: To investigate the changes of free triiodothyronine(FT₃) and free thyroxin(FT₄) in high lipid serum pretreated by polyethylene glycol(PEG).Methods: The specimen were divided into two groups:one was directly tested,the other was pretreated by PEG.FT₃ and FT₄ of the normal and high lipid serum were measured by radio immunoassay.Results: Compared with that directly tested in the normal group,the level of FT₃ and FT₄ had no statistic difference compared to the pretreated by PEG(P>0.05).The results of high lipid serum pretreated by PEG were markedly decreased compared to the directly tested(P<0.001).Conclusions: As the high lipid and thyroid hormone auto antibody is eliminated by PEG,the accuracy is improved.