汪强武, 王安潮, 陈余清. 端粒酶11-聚体寡核苷酸联合依托泊苷对肺癌细胞的作用[J]. 蚌埠医学院学报, 2005, 30(3): 208-210.
    引用本文: 汪强武, 王安潮, 陈余清. 端粒酶11-聚体寡核苷酸联合依托泊苷对肺癌细胞的作用[J]. 蚌埠医学院学报, 2005, 30(3): 208-210.
    WANG Qiang-wu, WANG An-chao, CHEN Yu-qing. Effect of telomerase 11-base oligodeoxynucleotides in combination with etoposide on the lung cancer cell line[J]. Journal of Bengbu Medical College, 2005, 30(3): 208-210.
    Citation: WANG Qiang-wu, WANG An-chao, CHEN Yu-qing. Effect of telomerase 11-base oligodeoxynucleotides in combination with etoposide on the lung cancer cell line[J]. Journal of Bengbu Medical College, 2005, 30(3): 208-210.

    端粒酶11-聚体寡核苷酸联合依托泊苷对肺癌细胞的作用

    Effect of telomerase 11-base oligodeoxynucleotides in combination with etoposide on the lung cancer cell line

    • 摘要: 目的: 探讨依托泊苷(VP-16)单独或联合端粒酶11-聚体寡核苷酸对肺腺癌细胞的作用。方法: VP-16单独或联合脂质体介导的端粒酶11-聚体寡核苷酸与肺腺癌细胞株共培养,MTT法检测细胞生长抑制率;流式细胞术检测细胞周期及细胞凋亡;以TRAP-ELISA法半定量测定细胞端粒酶活性。结果: VP-16抑制肺腺癌细胞株SPC-A1细胞生长,抑制率18.9%,下调端粒酶活性,与未加VP-16比较均有明显不同(P<0.01),可诱导细胞凋亡。VP-16联合反义寡核苷酸(ASODN)与联合正义寡核苷酸(SODN)对SPC-A1细胞的抑制率分别为43.5%和41.0%,两者差异无显著性(P>0.05);联合用药效果明显优于单用VP-16,差异有显著性(P<0.01)。结论: 化疗药联合短链正、反义核苷酸可抑制肺癌细胞生长,较二者单独应用可提高疗效,同时降低副作用。

       

      Abstract: Objective: To investigate the effect of etoposide(VP-16),in combination with oligodeoxynucleotides on lung adenocarcinoma cells.Methods: Etoposide in combination with telomerase 11-base oligodeoxynucleotides werer transfected into SPC-A1 cell line by lipofection.The multiparameters such as cell growth inhibition rate,cell cycle and apoptasis rate,and telomerase activity were detected by MTT,FCM,and PCR-ELISA,respectively.Results: Etoposide inhibited cell growth(with an inhibition rate of 18%),decreased telomerase activity and induced cell apoptosis.ASODN+VP-16 and SODN+VP-16 inhibit the growth of SPC-A1 cell line and rate are 43.5% and 41.0%,repectively(P>0.05).The difference was significant when compared with VP-16(P<0.01).Conclusions: Chemotherapeutant in combination with short chain oligodeoxynucleotides is more effective than used alone in inhibiting lung cancer and the side effect can be greatly reduced.

       

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