王玮, 陈立军, 初殿伟, 呼文亮. 人源肝癌细胞系中甲胎蛋白基因表达及甲基化的研究[J]. 蚌埠医学院学报, 2009, 34(2): 106-109.
    引用本文: 王玮, 陈立军, 初殿伟, 呼文亮. 人源肝癌细胞系中甲胎蛋白基因表达及甲基化的研究[J]. 蚌埠医学院学报, 2009, 34(2): 106-109.
    WANG Wei, CHEN Li-jun, CHU Dian-wei, HU Wen-liang. Methylation and expression of α-fetoprotein gene in human hepatocellular carcinoma cell line[J]. Journal of Bengbu Medical College, 2009, 34(2): 106-109.
    Citation: WANG Wei, CHEN Li-jun, CHU Dian-wei, HU Wen-liang. Methylation and expression of α-fetoprotein gene in human hepatocellular carcinoma cell line[J]. Journal of Bengbu Medical College, 2009, 34(2): 106-109.

    人源肝癌细胞系中甲胎蛋白基因表达及甲基化的研究

    Methylation and expression of α-fetoprotein gene in human hepatocellular carcinoma cell line

    • 摘要: 目的:了解不同人源肝癌细胞系甲胎蛋白(AFP)基因表达及不同区域甲基化状态,探讨肝癌发生的表达遗传学调控机制。方法:采用亚硫酸氢盐-基因组测序(bisulfite-assisted genomic sequencing,BAGS)技术,检测人源HepG2、SMMC-7721肝癌细胞系和人成纤维细胞中AFP基因启动子区及CpG岛区的甲基化水平,RT-PCR检测基因的表达,并分析相关性。结果:HepG2细胞高表达AFP,SMMC-7721细胞低表达AFP,成纤维细胞不表达AFP;在启动子区,HepG2细胞的非甲基化修饰位点发生率较高,尤其是第一个和第二个CG位点,SMMC-7721细胞的甲基化程度较之高,而成纤维细胞甲基化程度整体很高;在CpG岛区,成纤维细胞的非甲基化修饰位点发生率较高,主要是第6个和第7个CG位点,SMMC-7721、HepG2细胞的甲基化程度较之高。结论:AFP基因启动子甲基化程度与基因表达水平存在负相关,AFP基因CpG岛甲基化程度与基因是否表达有关,AFP基因启动子低甲基化可能是AFP高表达的分子机制。

       

      Abstract: Objective: To study the mechanism of development in hepatocellular carcinoma by analyzing the methylation patterns of α-fetoproteins(AFP) gene and its expression in human hepatocellular carcinoma cell line.Methods: To analyze the relationship of AFP gene methylation and its expression,the methylation status of the promoter and CpG island of AFP gene was investigated by means of bisulfite-assisted genomic sequencing in HepG2,SMMC-7721 and fibroblasts.AFP gene expression was detected by RT-PCR.Results: AFP gene was found expressed in HepG2 cell line higher,which in SMMC-7721 was high,and in fibroblasts was the lowest.In the promoter area of AFP gene,the methylation level was low in HepG2 cell line,which in SMMC-7721 was high,and in fibroblasts was the highest,especially at the first and the second CG sites.But in the CpG island of AFP gene,the methylation level was high in HepG2 and SMMC-7721 cell line,which in fibroblasts was low,especially at the sixth and the seventh CG sites.Conclusions: The methylation patterns of AFP gene may associated with the level of AFP gene expression,AFP gene silencing is associated with the promoter methylation.

       

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