张成斌, 燕善军, 王启之, 承泽农, 陶仪声. survivin基因检测在胃癌辅助诊断中的应用[J]. 蚌埠医学院学报, 2007, 32(6): 649-651,766.
    引用本文: 张成斌, 燕善军, 王启之, 承泽农, 陶仪声. survivin基因检测在胃癌辅助诊断中的应用[J]. 蚌埠医学院学报, 2007, 32(6): 649-651,766.
    ZHANG Cheng-bin, YAN Shan-jun, WANG Qi-zhi, CHENG Ze-nong, TAO Yi-sheng. Survivin gene in auxiliary diagnosis of gastric carcinoma[J]. Journal of Bengbu Medical College, 2007, 32(6): 649-651,766.
    Citation: ZHANG Cheng-bin, YAN Shan-jun, WANG Qi-zhi, CHENG Ze-nong, TAO Yi-sheng. Survivin gene in auxiliary diagnosis of gastric carcinoma[J]. Journal of Bengbu Medical College, 2007, 32(6): 649-651,766.

    survivin基因检测在胃癌辅助诊断中的应用

    Survivin gene in auxiliary diagnosis of gastric carcinoma

    • 摘要: 目的: 探讨survivin基因检测在胃癌辅助诊断中的应用价值。方法: 收集49例胃癌组织及相应的癌旁非瘤胃组织标本,分别采用RT-PCR和IHC两种方法检测胃癌和癌旁组织中survivin mRNA和survivin蛋白的表达。结果: 65.3%(32例)的胃癌组织survivin mRNA和63.3%(31例)的胃癌组织survivin蛋白表达阳性,而癌旁非瘤胃组织内survivin mRNA和survivin蛋白均无阳性表达;运用RT-PCR法较IHC法检测survivin表达的阳性率略高,但其差异无统计学意义(P>0.05)。结论: survivin在胃癌组织中表达上调,免疫组化和RT-PCR检测胃癌组织survivin基因的表达有望成为一种新的胃癌辅助诊断指标。

       

      Abstract: Objective: To explore the value of the expression of survivin gene in auxiliary diagnosis of gastric cancer.Methods: The gastric carcinoma tissues and the neighboring noncancerous tissues of 49 cases with histologically verified gastric carcinoma were obtained.The survivin mRNA level was measued by RT-PCR and the protein expression of survivin was detected by immunohistochemical assay.Results: Survivin mRNA proved positive in 65.3%(32 cases)of the gastric cancerous tissues and 63.3%(31cases)of the protein respectively.In contrast,no expression of survivin was detected in the corresponding noncancerous tissues.The expression of survivin gene detected by RT-PCR was a bit higher than IHC,with no statistical significance(P>0.05).Conclusions: The expression of survivin in gastric cancerous tissues is up-regulated.Detection of survivin gene by immunohistochemistry or RT-PCR would be a new method in diagnosis of gastric cancer.

       

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