三氧化二砷对恶性黑色素瘤A<sub>375</sub>细胞株凋亡的诱导作用

三氧化二砷对恶性黑色素瘤A₃₇₅细胞株凋亡的诱导作用

摘要: 目的: 研究三氧化二砷(As₂O₃)对人恶性黑色素瘤A₃₇₅细胞株凋亡的诱导作用。方法: 采用流式细胞术(FCM)检测不同浓度的三氧化二砷诱导A 375细胞的早期凋亡率和晚期凋亡率。结果: As₂O₃浓度分别为5 μmol/L、10 μmol/L、20 μmol/L时,A₃₇₅细胞的早期凋亡率分别为9.75%、50.9%和43.7%,晚期凋亡率分别为2.10%、4.61%和11.2%,总凋亡率分别为11.85%、55.51%和54.9%。结论: As₂O₃可诱导A₃₇₅细胞早期凋亡和晚期凋亡,10 μmol/L和20 μmol/L的As₂O₃可明显增加A₃₇₅细胞总凋亡率。

Abstract: Objective: To study the effect of arsenic trioxide on induction of apoptosis of melanoma A₃₇₅ cell line.Methods: Early apoptosis rate and late apoptosis rate in A₃₇₅ cells were analysed by flow cytometry.Results: After pretreated with arsenic trioxide of 5 μmol/L,10 μmol/L,and 20 μmol/L,the early apoptosis rate in A₃₇₅ cells were 9.75%,50.9%,and 43.7%,respectively.The late apoptosis rate in A₃₇₅ cells were 2.10%,4.61%,and 11.2%,respectively.The totle apoptosis rate were 11.85%,55.51%,and 54.9%,respectively.Conclusions: The early and the late apoptosis rate in A₃₇₅ cells were induced by arsenic trioxide.