Abstract: Objective: To investigate the anti-CCP and rheumatoid factor(RF) in the diagnosis of rheumatoid arthritis(RA) patients.Methods: Forty cases of patients with RA and 40 cases of patients with other autoimmune diseases and 40 health people(control group) were selected.The concentration of anti-CCP was determined by electrochemical luminescence analysis and the concentration of RF was determined by chemical analysis,diagnostic sensitivity and specificity of anti-CCP antibodies and RF in RA were compared.Results: The anti-CCP content in the serum of RA patients was found distinctly higher than the control group and non-RA group(P<0.01),control group and non-RA group showed no significant difference with the level of anti-CCP antibody(P>0.05).The sensitivity of anti-CCP antibody and RF was 97.50% and 83.75%,there was significant difference between them(P<0.01).The specificity of anti-CCP antibody and RF was 65.0% and 67.5%,there was no significant difference between them(P>0.05).The specificity and sensitivity of combined detection of anti-CCP and RF were 98.75% and 82.50%.Conclusions: Detection of anti-CCP in Serum has a great value for diagnosis of RA and could be an ideal serological data for diagnosis of RA.Combined detection of anti-CCP antibody and RF could increase the diagnostic specificity and sensitivity.