肝硬化大鼠肝移植后脾脏功能的变化

    Changes of spleen function of hepatic cirrhosis after liver transplantation in rats

    • 摘要: 目的: 探讨肝硬化大鼠肝移植后脾脏功能的变化。方法: 制备四氯化碳中毒性肝硬化大鼠模型,采用“二袖套法”进行肝移植。观察肝移植前后大鼠外周血细胞数、脾指数、门静脉压力以及脾脏组织形态学的变化。应用免疫荧光染色技术及流式细胞仪检测肝移植前后大鼠脾脏T淋巴细胞亚群的变化。结果: 肝移植前,肝硬化大鼠外周血细胞数均显著降低(P<0.01);脾脏病理切片示白髓面积缩小(P<0.01),脾小梁面积扩大(P<0.01);脾脏T淋巴细胞亚群CD4/CD8比值明显下降(P<0.01)。肝移植后,随着时间不断延长,大鼠外周血细胞数均明显回升(P<0.01);脾脏病理切片示白髓面积扩大(P<0.01);脾小梁面积有所缩小(P<0.05);脾脏T淋巴细胞亚群中CD4/CD8比值也有所升高(P<0.01)。结论: 肝硬化大鼠肝移植后脾功能亢进明显缓解,脾脏免疫功能异常也得到改善。

       

      Abstract: Objective: To investigate the changes of spleen function of hepatic cirrhosis rats after liver transplantation.Methods: Hepatic cirrhosis model was established in rats with subcutaneous injections of carbon tetrachloride.Liver transplantation model was established with two-cuff technique.The cell counts of peripheral blood and the morphological changes of spleen were observed before and after liver transplantation in hepatic cirrhosis rats.The spleen T lymphocyte subsets before and after liver transplantation were also assayed by using immunofluorescence staining and flow cytometry.Results: Before liver transplantation,the cell counts in peripheral blood of hepatic cirrhosis rats were significantly decreased(P<0.01).The pathological sections of spleen showed that the areas of white pulp were decreased(P<0.01),and the areas of spleen trabecula were increased(P<0.01).Meanwhile,the ratio of CD4/CD8 of spleen T lymphocyte subsets was decreased(P<0.01)while after liver transplantation,the cell counts of peripheral blood were significantly increased(P<0.01).The pathological sections of spleen showed that the areas of white pulp were increased(P<0.01),and the areas of spleen trabecula were decreased(P<0.05).Meanwhile,the ratio of CD4/CD8 of spleen T lymphocyte subsets was increased(P<0.01).Conclusions: Hypersplenism of hepatic cirrhosis rats could be significantly relieved after liver transplantation.Liver transplantation can improve the impaired function of spleen.

       

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