Abstract: Objective: To constrct the eukaryotic expression vector of interleukin-18(IL-18) and study its expression in HO-8910 cell line.Methods: Interleukin-18 gene was inserted into the eukaryotic expression vector pCI with molecular cloning technique,transferred to HO-8910 cell line with Lipotectamine.Three transferred groups were studied:HO-8910+Lipotectamine,HO-8910+pCI and HO-8910 + pCI-hIL-18.The expression of IL-18 gene was comfirmed by RT-PCR technique.Results: The eukaryotic expression vector of IL-18(pCI-hIL-18) was successfully constructed and transferred to HO-8910 cells and expressed in mRNA and protein level of the HO-8910 cell line,but not expressed in the other two groups.Conclusions: The IL-18 gene could be expressed in the HO-8910 cell line.