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    吴晓莉, 张林杰, 许健. IL-18真核表达载体的构建及其在HO-8910细胞株的表达[J]. 蚌埠医科大学学报, 2008, 33(2): 144-146.
    引用本文: 吴晓莉, 张林杰, 许健. IL-18真核表达载体的构建及其在HO-8910细胞株的表达[J]. 蚌埠医科大学学报, 2008, 33(2): 144-146.
    shu
    WU Xiao-li, ZHANG Lin-jie, XU Jian. Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line[J]. Journal of Bengbu Medical University, 2008, 33(2): 144-146.
    Citation: WU Xiao-li, ZHANG Lin-jie, XU Jian. Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line[J]. Journal of Bengbu Medical University, 2008, 33(2): 144-146.
    shu

    IL-18真核表达载体的构建及其在HO-8910细胞株的表达

    Constructing of interleukin-18 eukaryotic expression vector and expression of interleukin-18 in HO-8910 cell line

      摘要
    • 摘要: 目的:通过构建人白细胞介素18(IL-18)真核表达载体,观察其在HO-8910细胞株的表达情况。方法:应用分子克隆技术将IL-18基因插入pCI真核表达载体,应用脂质体介导法转染人卵巢癌HO-8910细胞株。转染分3组,空白组:人卵巢癌HO-8910细胞株+脂质体;对照组:卵巢癌HO-8910细胞株+pCI;实验组:卵巢癌HO-8910细胞株+pCI-hIL-18。以RT-PCR方法分别检测3组的IL-18表达情况。结果:成功构建pCI-hIL-18真核表达载体并转染HO-8910细胞株,RT-PCR、ELISA显示转染pCI-hIL-18重组质粒组在转染的细胞内获得表达。结论:IL-18基因能够转染到HO-8910细胞株中并获得表达。

       

      Abstract: Objective: To constrct the eukaryotic expression vector of interleukin-18(IL-18) and study its expression in HO-8910 cell line.Methods: Interleukin-18 gene was inserted into the eukaryotic expression vector pCI with molecular cloning technique,transferred to HO-8910 cell line with Lipotectamine.Three transferred groups were studied:HO-8910+Lipotectamine,HO-8910+pCI and HO-8910 + pCI-hIL-18.The expression of IL-18 gene was comfirmed by RT-PCR technique.Results: The eukaryotic expression vector of IL-18(pCI-hIL-18) was successfully constructed and transferred to HO-8910 cells and expressed in mRNA and protein level of the HO-8910 cell line,but not expressed in the other two groups.Conclusions: The IL-18 gene could be expressed in the HO-8910 cell line.

       

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