短链脂肪酸作用下伤寒沙门菌对巨噬细胞凋亡的影响

Macrophage apoptosis induced by short-chain fatty acid stressed Salmonella typhi

摘要: 目的:了解伤寒沙门菌及其短链脂肪酸(short-chain fatty acid,SCFA)作用菌诱导人巨噬细胞凋亡的差异。方法:使用流式细胞术检测SCFA作用菌及原菌诱导巨噬细胞2、4、8、12及24h的细胞凋亡率。结果:SCFA作用菌及原菌均能诱导巨噬细胞发生凋亡,与对照组比较差异均有统计学意义(P<0.01);SCFA作用组不同时间诱导细胞凋亡率均高于原菌组(P<0.01)。结论:SCFA可促进伤寒沙门菌诱导巨噬细胞凋亡。

Abstract: Objective: To compare the difference of the apoptosis of macrophage induced by Salmonella typhi(S.typhi) and that treated by short-chain fatty acid(SCFA).Methods: The apoptosis ratio of macrophage at different times after infection with S.typhi or its SCFA forms were determined by flow cytometry.Results: Both S.typhi and its SCFA forms induced apoptosis of the macrophage with significant difference in the rates of apoptosis compared with those of the control(P<0.01).However,the rate of apoptosis induced by SCFA forms was obviously higher than that by S.typhi(P<0.01).Conclusions: SCFA may promote the apoptosis of macrophage.