Abstract: Objective: To explore the clinical value of the percutaneous biopsy guided by CT in the diagnosis of pulmonary aspergillosis.Methods: The clinical data of 34 patients with pulmonary aspergillosis treated with percutaneous paracentesis guided by CT were retrospectively analyzed.Results: Thirty cases with pulmonary aspergillosis were diagnosed by biopsy,4 cases with undiagnosed pulmonary aspergillosis were identified by the surgical pathology.The clinical manifestation showed cough in 25 cases,expectoration in 20 cases,fever in 18 cases,emptysis in 6 cases,chest pain in 5 cases,dyspnea in 5cases and no symptom in 3 cases.The imaging showed nodule in 15 cases(single in 6 cases and multiple in 9 cases),lump in 7 cases,consolidation in 5 cases,infiltrate in 4 cases and air-crescent sign in 3 cases.The positive rate of galactomannan test was 67.6%(23/34).The necrotizing inflammation was the mainly pathological characteristics.The pneumothorax rate was 17.6%(6/34),only one patient was treated with intrathoracic drain,and the others were automatically absorbed.Both 5 cases of needle passage hemorrhage and 1 case of hemoptysis alleviated without treatment.Conclusions: The percutaneous biopsy guided by CT is a safe and effective means in diagnosing pulmonary aspergillosis.