实时荧光定量PCR检测乙型肝炎病毒DNA的临床应用价值

Real-time fluorescence quantitative PCR in determination of hepatitis B virus DNA

摘要: 目的: 探讨实时荧光定量PCR(F Q-PCR)检测乙型肝炎病毒DNA的临床应用价值。方法: 采用FQ-PCR方法检测196例患者血清中HBV-DNA。结果: 112例HBeAg(+)/HBeAb(-)标本中FQ-PCR均阳性,HBV-DNA拷贝数1.08×10⁷/ml;68例HBeAg(-)/HBeAb(+)标本中,平均拷贝数6.12×10⁵/ml,其阳性率为66.2%;16例HBeAg(-)/HBeAb(-)标本中,平均拷贝数为2.72×10⁴/ml。结论: 实时FQ-PCR可以实现准确定量,是了解HBV在体内复制和判断疗效的有利手段。

Abstract: Objective: To explore the role of the real time fluorescence quantitative polymerase chain reaction (FQ PCR) in detecting heptitis B virus (HBV) DNA.Methods: The sera from 196 patients were measured by FQ PCR.Results: In the 112 HBeAg (+)/HBeAb (-) samples,the FQ PCR results were all positive,with 1.08×10⁷/ml of HBV DNA on the average.In the 68 HBeAg (-)/HBeAb (+) samples,the positive rate was 66.2% with a duplication amount of 6.12×10⁵/ml on the average.In the 16 HBeAg (-)/HBeAb (-) samples,the duplication amount was 2.72×10⁴/ml.Conclusions: FQ PCR can ensure the accurate quantity.It is a useful method in understanding HBV duplication and evaluating the therapeutic effect.