Abstract: Objective: To explore the pathogenic bacteria distribution of bone and joint purulent infection and its drug resistance for providing the basis of antimicrobial treatment.Methods: The pathogenic bacteria from 418 samples of patients with bone and joint purulent infection were isolated and identified,and the antibiotics sensitivity tests were examined.Results: Two hundred and twelve strains gram-positive bacteria and 176 gram-negative bacteria were isolated form 418 samples.The detection rates of Staphylococcus aureus,Pseudomon asaeruginosa,Escherichia coli,Proteus mirabilis and Staphylococcus epidermidis were 38.28%,11.48%,8.13%,5.26% and 4.78%,respectively.Conclusions: The drug-resistant Staphylococcus aureus is the mainly pathogenic bacteria of bone and joint purulent infection.The antibiotics treatment should be taken according to the results of antibiotics sensitivity tests which can induce the resistance to antibiotics.