Abstract: Objective:To explore the changes of pulmonary ventilation function(PVF) in obese rats with leptin resistance induced by high-fat diet. Methods:Thirty female Sprague-Dawley weaning rats were randomly divided into control group and high-fat diet group according to their body weight. Fifteen rats were fed with basic diet and 15 rats were fed with high-fat diet. At the end of the 8th week, 7 obese rats were selected by body weight from high-fat diet group as obese group with leptin resistance. The body weight,blood serum leptin concentration,tidal volume(TV),pulmonary ventilation(PV),forced vital capacity(FVC),maximal midexpiratary flow curve (MMEF),peak inspiratory flow(PIF) and peak expiratory flow(PEF) of all rats in two groups were detected. Results:The body weight from the 3rd week to the 8th week,blood serum leptin concentration,PV,PIF and PEF of obese group at the end of the 8th week were higher than those of control group(P<0.05-P<0.01). No significant difference of TV,FVC and MMEF were found between two groups at the end of 8th week. Conclusions:Obesity with leptin resistance induced by high-fat diet impairs the PVF of female rats.