AC-88对荷瘤小鼠抑瘤作用及对T细胞Fas蛋白表达的影响

    The antitumor effect of AC-88 in mice bearing cancer and its effect on T cell Fas expression

    • 摘要: 目的: 探讨AC-88的抑瘤作用和可能机制。方法: 以移植H22肝癌细胞、艾氏腹水瘤细胞(EAC)和Meth A肉瘤细胞的荷瘤小鼠为体内实验模型,以培养的人大肠癌细胞株(LO VO)、人卵巢癌细胞株(TYK)和H22细胞为体外模型,研究AC-88对肿瘤细胞的抑制作用,并测定荷瘤小鼠的T细胞Fas蛋白的表达。结果: 给三种荷瘤小鼠灌服AC-88 250 mg/kg组的体内抑瘤率分别高达76.31%、73.52%和71.91%,平均瘤重均低于对照组(P<0.01);体外的半数抑制浓度IC50均小于10μg/ml,而且AC-88能降低荷瘤小鼠的T细胞Fas蛋白的表达(P<0.01)。结论: AC-88有良好的抑瘤作用,这种作用可能与AC-88能降低T细胞Fas蛋白的表达有关。

       

      Abstract: Objective: To investigate the antitumor effect and possible mechanism of AC-88.Methods: H22,Meth A,EAC cell bearing mice in vivo and LOVO,TYK,H22 culture cells in vitro were used to study the antitumor effect of AC-88 and Western-blotting was used to study Fas expression of T lymphocytes.Results: The antitumor rate of AC-88 250 mg/kg group on three types tumor bearing mice was 76.31%,73.52%,and 71.91%,respectively.The average tumor weight was lower than control group(P<0.01).The IC 50 of AC-88 on each type tumor cells was lower than 10 μg/ml in vitro.Fas expression of T cells was decreased in the same time(P<0.01).Conclusions: AC-88 has obvious antitumor effect and maybe through decrease T cell Fas expression.

       

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