Abstract: Objective: To investigate the effect of reduced glutathione on oxidative stress in diabetic ketoacidosis(DKA) patients. Methods: Forty-seven cases of DKA patients were divided into two groups randomly:conventional treatment group and plus reduced glutathione treatment group. The levels of superoxide dismutase(SOD),total antioxidant capacity(TAC),malondialdehyde(MDA), advanced oxidation protein products(AOPP) of the two groups were compared before and after treatment. Results: After treatment by plus reduced glutathione,the content of SOD,TAC were increased significantly when compared with that in the conventional group(P < 0.01),however,the level of AOPP was decreased significantly(P < 0.01). Conclusions: Reduced glutathione could reduce the level of oxidative stress in DKA,and thus protect the body.