Abstract: Objective: To investigate the distribution and resistance of fungus in order to provide basis for antifungal clinical treatment. Methods: Microbiological assay and drug sensitive test were performed on 209 strains of clinical lyisolated separated fungus in the first people hospital of Chaohu city from Jan. to Dec. in 2010. Results: Two hundred and nine fungal strains were detected from various clinical submitted specimens in our hospital and Candida albicans had the highest detection rate of 70.82% (148 strains), followed by C. tropicalis with 14.35% (30 strains). The largest isolation rate of 67.46% was in sputum specimens. In the age distribution patients above 60 years old had the largest portion. In vitro antifungal susceptibility test revealed that fungi had higher sensitivity(> 98%) to 5 fluorocytosine and amphotericin B, slighter sensitivity to azole drugs (79.32%). Conclusions: In fungal infections,candida albicans,and candida tropicalis,are more common,and the detection rate of drug resistant strains shows upward trend,suggesting that the use of antibiotics in clinic should be prudent to reduce the drug resistant strains.