Abstract: Objective:To investigate the antibiotics use in a 3A grade hospital, and to provide reference for rational clinical antibiotics use and scientifically establish procurement plan.Methods:Original antibiotics drug's data from the Hospital Information System of this hospital from 2009 to 2010 were collected, defined daily dose, sales amount sort and defined daily dose were used to analyze the data.Results:Comparing 2010 to 2009, the species of antibiotics drug were decreased by 13.64%, sale amounts were increased by 6.01%;Oral administration clarithromycin sustained release tablet, cefdinir capsule, and cefetamet pivoxil tablet were the top three sales amount in 2 consecutive years.Cefotiam for injection, aztreonam, cefathiamidine were the top three sales amount in 2 consecutive years.Conclusions:In the past two years, the varity number of antibiotics was decreased notably, and the ratio of antibiotics to total drugs was reasonable.The authors advise that continue to strengthen propagand of rational antibiotics use, strictly follow “Guiding Principles for Clinical Application of Antibacterial”, and reduce irrationally drug use phenomenon.