归红祛风酒微生物限度检查的方法学验证研究

Validation of the microbial limit inspection method of guihongqufeng wine

摘要: 目的:建立归红祛风酒的微生物限度检查方法。方法:利用薄膜过滤消除归红祛风酒中的絮状沉淀。分别采用培养基稀释法、薄膜过滤法对样品进行微生物限度检查;根据6株阳性对照菌的实验组、菌液组、供试品对照组的结果进行方法学验证。结果:大肠埃希菌、金黄色葡萄球菌、枯草芽孢杆菌、白色念珠菌和黑曲霉的菌落回收率均 >70%;通过控制菌检查,阳性实验菌均能检出。结论:薄膜过滤法能够适应菌落计数,归红祛风酒按照供试液制备法和控制菌检查法均能检出实验菌。

Abstract: Objective:To establish the method of the microbial limit inspection of guihongqufeng wine.Methods:The flocculent precipitation of preparation was removed by membrane-filter procedure.The microbial limit of sample was inspected by culture medium dilution and membrane-filter procedure.The methodology was validated by the results of 6 positive bacteria experimental group,microbial group and control group.Results:The recoverys of bacterial colony in Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Candida albicans and Aspergillus niger were more than 70%.All positive bacteria could be detected by the control germ test.Conclusions:The membrane-filter procedure can count the bacterial colony,the test organisms can be checked out by the methods of liquid preparation and control germ test.