鼻咽癌细胞株中乳腺癌转移抑制基因表达的研究

徐洪波; 孙谦; 汪庚明; 江浩; 张亚军; 周育夫

doi:10.13898/j.cnki.issn.1000-2200.2015.04.004

鼻咽癌细胞株中乳腺癌转移抑制基因表达的研究

The expression of breast cancer metastasis suppressor 1 in nasopharyngeal carcinoma cell lines

摘要

摘要: 目的:检测乳腺癌转移抑制(BRMS1)基因在不同转移习性的人鼻咽癌细胞株中的表达情况。方法:应用免疫组织化学法和逆转录聚合酶链式反应法在mRNA和蛋白2方面检测人鼻咽癌细胞株高分化磷癌、低分化磷癌、低分化细胞株、高成瘤高转移性细胞株、低成瘤低转移性细胞株中BRMS1的表达。结果:5种细胞株中BRMS1的蛋白表达皆为阳性,在不同转移习性的细胞株中其表达强度明显不同。4种细胞株中扩增出BRMS1 mRNA,而BRMS1 mRNA在高转移性细胞株中未扩增出,高分化鳞癌、低分化鳞癌、低分化细胞株BRMS1mRNA表达水平均显著高于低成瘤低转移性细胞株(P<0.01)。结论:BRMS1的表达与细胞株的转移能力有明显相关性,表明BRMS1可能作为鼻咽癌细胞转移的重要指标。

Abstract: Objective:To detect the expressions of breast cancer metastasis suppressor 1(BRMS1) gene in different human nasopharyngeal carcinoma(NPC) cell lines.Methods:The expressions of BRMS1 protein and mRNA in SUNE-1-5-8F, CNE-1, CNE-2Z, SUNE-1 and SUNE-1-6-10B of NPC cell lines were detected by immunohistochemical method and RT-PCR, respectively.Results:The positive expressions of BRMS1 protein in CNE-1, CNE-2Z, SUNE-1, SUNE-1-5-8F and SUNE-1-6-10B cell lines were identified.Except the SUNE-1-5-8F cell line, the mRNA expressions of BRMS1 in other four NPC cell lines were positive.The expression levels of BRMS1 mRNA in CNE-1, CNE-2Zand SUNE-1 cell lines were significantly higher than that in SUNE-1-6-10B cell line.Conclusions:The expression of BRMS1 is correlated to the metastatic ability of cell line, which may be an important marker in identifying the metastasis of NPC.

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