Abstract: Objective:To explore the clinical effects of the application of whole seamless nursing model in operation room. Methods: Seventy-four patients scheduled by operation were randomly divided into the control group and intervention group(37 cases each group). The control group and intervention group were nursed with the quality nursing and whole seamless nursing model based on the quality nursing, respectively. The mental comfort, surgical stress and nursing satisfaction in two groups were observed before and after nursing. Results:Compared with before nursing, the SAS and SDS scores in two groups were significantly decreased after nursing(P<0.01), but for intervention group, the SAS and SDS scores decreased more significantly(P<0.01). After nursing, the systolic and diastolic pressure and heart rate in intervention group were significantly lower than those in the control group(P<0.01). The difference of the satisfaction in the two groups was not statistically significant(P>0.05). Conclusions:The application of whole seamless nursing model in operation room can effectively improve the patient's mental comfort, reduce the occurrence of surgical stress and make the patients maintain a good attitude.