Abstract: Objective: To analyze the T-lymphocyte subsets change in peripheral blood of patients with chronic hepatitis B(CHB), and its correlation with hepatitis B-deoxyribonucleic acid(HBV-DNA). Methods: The relative literatures of T-lymphocyte subsets change in peripheral blood of patients with CHB from Medline(PubMed retrieval platform), Embase(Ovild retrieve platform), Central(Ovild retrieval platform), Whip, Wanfang and CNKI from January 1, 2000 to December 31, 2013 were searched. Independent screening documents, extracting information and evaluation were implemented independently by 2 evaluators according to the inclusion and exclusion criteria, which was analyzed by Meta using RevMan 5.3 software. Results: The percentage of CD3⁺T, CD4⁺T and CD4⁺T/CD8⁺T in patients with CHB were lower than those in healthy people, but the percentage of CD8⁺T cells of patients was higher(P<0.05). Compared with the HBeAg(-) patients, the percentage of CD4⁺T and CD8⁺T decreased and increased significantly in HBeAg(+) patients, respectively(P<0.01), the difference of CD4⁺T/CD8⁺ between HBeAg(-) and HBeAg(+) patients was not statistical significance(P>0.05). Compared with the negative HBV DNA patients, the percentage of CD3⁺T, CD4⁺T and CD4⁺T/CD8⁺T, and percentage of CD8⁺T decreased and increased significantly in the positive HBV DNA patients, respectively(P<0.01). Compared with the low copies of HBV DNA patients, the percentage of CD4⁺T and CD4⁺T/CD8⁺T and percentage of CD8⁺T decreased and increased significantly in high copies of HBV DNA patients, respectively(P<0.01), the difference of percentage of CD3⁺T between the low and high copies of HBV DNA patients was not statistical significance(P>0.05). Conclusions: HBV infection can lead to the change of T cell subsets of patients, which is positively related to the HBV-DNA level.