Abstract: Objective: To compare the effects of 3 kinds of different amount root canal filler in the treatment of dental pulp. Methods: One hundred and two patients(146 teeth) were treated with root canal filler. After treatment, these teeth were divided into the suitable supplement group(109 teeth), excessive supplement group(14 teeth) and deficient supplement group(23 teeth) according to the imaging data. The clinical effects and pain incidence between 3 groups were compared. Results: The total efficiency rare in suitable supplement group(94.50%) was significantly higher than that in excessive supplement group(64.29%) and deficient supplement group(47.83%)(P<0.01). The pain incidences in suitable supplement group, excessive supplement group and deficient supplement group were 3.67%, 14.29% and 26.09%, respectively, the differences of those between 3 groups were not statistically significant(P>0.05). Conclusions: Among the three kinds of different amount root canal filler, the treatment effect of suitable supplement is best, and the excessive and deficient supplement method should not be used in clinic.