Abstract: Objective: To explore and practice the protein and enzyme engineering three-dimensional teaching according to the cultivation of consciousness of innovation talents.Methods: The protein and enzyme engineering three-dimensional teaching model was carried out in class 2013 of biological sciences of Bengbu Medical College,the teaching effect was compared with the traditional teaching method used in class 2012.Results: The ability of independent learning,consciousness of innovation,spirit of teamwork,teaching effects and assessment of learning quality in the student of class 2013 executed three-dimensional teaching model were significantly better than those in class 2012(P < 0.01).Conclusions: The effects of three-dimensional teaching model on the effect of teaching effects,especially on autonomous learning ability,application ability and consciousness of innovation talents are better than those whose of traditional teaching,which is beneficial to the cultivation of innovative and practical medical talents.