钙蛋白酶抑制剂Calpeptin对乳腺癌MDA-MB-231细胞迁移的影响

张梦晓; 孙小锦; 张智瑞; 潘琼; 赵素容; 刘浩

doi:10.13898/j.cnki.issn.1000-2200.2016.07.001

钙蛋白酶抑制剂Calpeptin对乳腺癌MDA-MB-231细胞迁移的影响

Effect of calpain inhibitor Calpeptin on the migration of MDA-MB-231 breast cancer cells

摘要

摘要: 目的：探讨钙蛋白酶抑制剂Calpeptin对乳腺癌MDA-MB-231细胞迁移能力的影响，为治疗乳腺癌转移提供思路。方法：MTT法检测不同浓度Calpeptin对MDA-MB-231细胞增殖的影响；细胞划痕实验及Transwell迁移实验检测不同浓度的Calpeptin对MDA-MB-231细胞迁移的影响；Western blot法检测不同浓度Calpeptin对MDA-MB-231细胞基质金属蛋白酶-2表达的影响。结果：Calpeptin可抑制MDA-MB-231细胞的增殖，其增殖抑制作用随浓度的增大而增加（P<0.01）。Calpeptin作用MDA-MB-231细胞24 h后，细胞的迁移能力明显下降（P<0.01）。Western blot结果显示，Calpeptin可下调MDA-MB-231细胞中细胞基质金属蛋白酶-2的表达。结论：Calpeptin能够抑制乳腺癌MDA-MB-231细胞迁移，其机制可能与下调MMP-2的表达有关。

Abstract: Objective: To investigate the effects of calpain inhibitor Calpeptin on the migration of MDA-MB-231 breast cancer cells for providing an idea in treating the metastatic breast cancer.Methods: The effects of different concentrations of Calpeptin on the proliferation of MDA-MB-231 cells were determined using MTT assay.The effects of different concentrations of Calpeptin on the migration of MDA-MB-231 cells were detected using the wound healing assay and transwell migration assay.The effects of different concentrations of Calpeptin on the expression of matrix metalloproteinase-2(MMP-2) in MDA-MB-231 cells were detected using Western blot.Results: Calpeptin could suppress the proliferation of MDA-MB-231 breast cancer cells,the inhibiting effects of which increased with the increasing of the concentrations of Calpeptin(P<0.01).The migration ability of MDA-MB-231 cells decreased significantly after 24 h of treatment with Calpeptin(P<0.01).The result of Western blot showed that Calpeptin could down-regulate the expression of MMP-2 in MDA-MB-231 cells.Conclusions: Calpeptin can inhibit the migration of MDA-MB-231 cells,the mechanism of which may involve in the down-regulation of MMP-2.

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