长链非编码RNA GAS5对人乳腺癌细胞株增殖和侵袭能力的影响

吴海华; 李钰; 张凌宇; 王海凤; 陈丽; 黄文明; 陈素莲; 陈昌杰; 杨清玲

doi:10.13898/j.cnki.issn.1000-2200.2016.07.003

长链非编码RNA GAS5对人乳腺癌细胞株增殖和侵袭能力的影响

The effect of LncRNA GAS5 on prolification, migration and invation in breast cancer cells

摘要

摘要: 目的：探讨长链非编码RNA GAS5对人乳腺癌细胞增殖、侵袭及迁移生物学活性的影响。方法：qRT-PCR方法检测3株乳腺癌细胞株SKBR-3、 MDA-MB-231及MCF-7中LncRNA GAS5的表达；LncRNA GAS5干扰片段和过表达载体分别转染LncRNA GAS5高表达以及低表达的乳腺癌细胞株，qRT-PCR方法检测转染后LncRNA GAS5的表达；磺酰罗丹明B染色法检测细胞的增殖能力；Transwell和划痕实验检测细胞的侵袭及迁移能力。结果：LncRNA GAS5在乳腺癌SKBR-3细胞中表达量最低，MCF-7细胞中表达量最高（P<0.01）；SKBR-3以及MCF-7细胞分别转染LncRNA GAS5过表达载体和干扰片段后，SKBR-3细胞LncRNA GAS5表达量增高，MCF-7表达量降低（P<0.01）；下调LncRNA GAS5的表达，细胞的增殖能力升高，侵袭及迁移能力增强（P<0.01）；过表达LncRNA GAS5之后，细胞的增殖能力、侵袭和迁移能力减弱（P<0.01）。结论：LncRNA GAS5是涉及到乳腺癌发展的一个新型的分子，有可能成为乳腺癌治疗的潜在靶点。

Abstract: Objective: To investigate the effect of LncRNA GAS5 on prolification,migration and invation in breast cancer cells.Methods: To achieve our goal,we used qRT-PCR assay to detect the expression of LncRNA GAS5 in breast cancer SKBR-3,MDA-MB-231 and MCF-7 cells;SRB assay was used to measure the prolification ability of breast cancer cells;Wound healing and Transwell assay was used to dentect the ability of invation and migration in breast cancer cells.Results: Among the three breast cancer cell lines,the expression level of LncRNA GAS5 in SKBR-3 cell is lowest and the expression level of LncRNA GAS5 in MCF-7 cell is highest(P<0.01).After transfected MCF-7 or SKBR-3 cells with si-GAS5 or pcDNA-GAS5-vertor,the expression level of LncRNA GAS5 in MCF-7 cells is down-regulated and in SKBR-3 cells is up-regulated(P<0.01),depletion of LncRNA GAS5,the prolification,invation and migration ability of MCF-7 cells is increased(P<0.01).Overexpression of LncRNA GAS5 made the prolification,invation and migration ability of SKBR-3 cells decreased(P<0.01).Conclusions: lncRNA GAS5 is a novel molecule involved in breast cancer progression,which provide a potential therapeutic target.

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